Classification and application of metal-based nanoantioxidants in medicine and healthcare

• Nguyen Nhat Nam,
• Nguyen Khoi Song Tran,
• Tan Tai Nguyen,
• Nguyen Ngoc Trai,
• Nguyen Phuong Thuy,
• Hoang Dang Khoa Do,
• Nhu Hoa Thi Tran and
• Kieu The Loan Trinh

Beilstein J. Nanotechnol. 2024, 15, 396–415, doi:10.3762/bjnano.15.36

• serum albumin (HSA). (b) Organ distribution of EeNA and HSA

• thickness, Ce3+ concentration, and the resulting strain in ceria with specific surface orientation (100). Figure 2 was adapted with permission from [32]. Copyright 2023 American Chemical Society. This content is not subject to CC BY 4.0. (a) Percent injected dose (%ID) in blood of EaNA and Cy5-labeled human

Fluorescent bioinspired albumin/polydopamine nanoparticles and their interactions with Escherichia coli cells

• Eloïse Equy,
• Jordana Hirtzel,
• Sophie Hellé,
• Béatrice Heurtault,
• Eric Mathieu,
• Morgane Rabineau,
• Vincent Ball and
• Lydie Ploux

Beilstein J. Nanotechnol. 2023, 14, 1208–1224, doi:10.3762/bjnano.14.100

• and Ball [14] and Bergtold et al. [13], human serum albumin and other proteins play an important role in size control and stability of PDA NPs. Similarly, the stability of BSA/PDA NPs is probably due to BSA thanks to the strong PDA/KE interactions reported by Bergtold et al. This is supported by the

Curcumin-loaded albumin submicron particles with potential as a cancer therapy: an in vitro study

• Nittiya Suwannasom,
• Netsai Sriaksorn,
• Chutamas Thepmalee,
• Krissana Khoothiam,
• Ausanai Prapan,
• Hans Bäumler and
• Chonthida Thephinlap

Beilstein J. Nanotechnol. 2023, 14, 1127–1140, doi:10.3762/bjnano.14.93

• industrial manufacturing and application and has therefore attracted researchers [21]. Moreover, because of its various drug-binding sites, drugs can effectively be incorporated into the hydrophobic or hydrophilic core of albumin particles. It has been demonstrated that human serum albumin (HSA) acts as a

• effective delivery system, our second aim was to evaluate the cytotoxicity effect of CUR-HSA-MPs in vitro and determine cell uptake in MCF-7 cell line. Results and Discussion Preparation of CUR-loaded human serum albumin microparticles The preparation process of CUR-loaded human serum albumin microparticles

• isothiocyanate (FITC) was purchased from Biochrom GmbH (Berlin, Germany). Fabrication of CUR-loaded human serum albumin microparticles The CUR-HSA-MPs were prepared by the CCD technique with some modifications to the adsorption method [29]. In brief, 20 mL of 0.25 M MnCl2 containing 50 mg/mL HSA solution were

Recognition mechanisms of hemoglobin particles by monocytes – CD163 may just be one

• Jonathan-Gabriel Nimz,
• Pichayut Rerkshanandana,
• Chiraphat Kloypan,
• Ulrich Kalus,
• Saranya Chaiwaree,
• Axel Pruß,
• Radostina Georgieva,
• Yu Xiong and
• Hans Bäumler

Beilstein J. Nanotechnol. 2023, 14, 1028–1040, doi:10.3762/bjnano.14.85

• chloride was purchased from B. Braun SE, Melsungen, Germany. Phosphate-buffered saline (PBS) 10-fold solution was purchased from Fisher scientific, Fair Lawn, New Jersey, USA. Human serum albumin (Plasbumin®20) was purchased from Grifols Deutschland GmbH, Frankfurt am Main, Germany. Trypan blue 0.4

• technique previously described [24] has been followed: 0.5% bovine hemoglobin solution was mixed with 0.125 M MnCl2 solution. While stirring rapidly, 0.125 M Na2CO3 was added and stirred further for 30 s to let Hb–MnCO3 particles form. 20% human serum albumin (HSA) solution was added and allowed to incubate

• the case of our HbMPs containing human serum albumin, we assume that a conformational change within HSA might occur when the molecules are cross-linked by glutaraldehyde. Detection of this denatured protein by receptors of phagocytic cells would also be conceivable in this case to explain CD204’s part

Design of surface nanostructures for chirality sensing based on quartz crystal microbalance

• Yinglin Ma,
• Xiangyun Xiao and
• Qingmin Ji

Beilstein J. Nanotechnol. 2022, 13, 1201–1219, doi:10.3762/bjnano.13.100

• serum albumin (SA) [31]. They evaluated the detection efficiencies of ten enantiomers, which were tetrahydronaphthylamine (TNA), 1-(4-methoxyphenyl)ethylamine (4-MPEA), 1-(3-methoxyphenyl)ethylamine (3-MPEA), 2-octanol (2-OT), and methyl lactate (MEL). The fixation of BSA and human serum albumin (HSA

Systematic studies into uniform synthetic protein nanoparticles

• Nahal Habibi,
• Ava Mauser,
• Jeffery E. Raymond and
• Joerg Lahann

Beilstein J. Nanotechnol. 2022, 13, 274–283, doi:10.3762/bjnano.13.22

• , particularly as a combination therapy [10]. To leverage the endogenous properties of albumin, nab technology uses a high-pressure manufacturing process to force hydrophobic drugs into the internal hydrophobic pockets of human serum albumin (HSA) [11]. This leads to the formation of albumin-bound, paclitaxel

• Discussion Single-protein SPNPs A range of SPNP formulations were prepared via EHD jetting from hemoglobin (HEM), transferrin (TF), mucin (MUC), insulin (INS), and human serum albumin (HSA) (Figure 1a). Generally, dilute solutions of a protein at 10% (w/v) in a 9:1 (v/v) mixture of water and ethanol were

• electrohydrodynamic co-jetting method will likely involve setups with multiple parallel jets [34]. Experimental Materials Recombinant human serum albumin was purchased from InVitria. Mucin from porcine stomach, human transferrin, human hemoglobin, recombinant human insulin, and O,O′-bis[2-(N-succinimidylsuccinylamino

Bacterial safety study of the production process of hemoglobin-based oxygen carriers

• Axel Steffen,
• Yu Xiong,
• Radostina Georgieva,
• Ulrich Kalus and
• Hans Bäumler

Beilstein J. Nanotechnol. 2022, 13, 114–126, doi:10.3762/bjnano.13.8

• modifications [5][6]. Thus, it was possible to immobilize vitamin B2 (riboflavin) in these particles together with human serum albumin (HSA). This resulted in a drug delivery system with good hemocompatibility and release of riboflavin over a prolonged period [7]. In addition, HSA microparticles could be loaded

• , Bernburg, Germany; aqua ad iniectabilia (Ampuwa) from Fresenius Kabi Deutschland GmbH, Bad Homburg, Germany, and sodium chloride (NaCl) from B. Braun Melsungen AG, Melsungen, Germany. Human serum albumin, 200 g/L, Baxalta was obtained from Takeda Pharma Vertrieb GmbH & Co. KG, Berlin, Germany. EDTA, PBS

Comprehensive review on ultrasound-responsive theranostic nanomaterials: mechanisms, structures and medical applications

• Sepand Tehrani Fateh,
• Lida Moradi,
• Elmira Kohan,
• Michael R. Hamblin and
• Amin Shiralizadeh Dezfuli

Beilstein J. Nanotechnol. 2021, 12, 808–862, doi:10.3762/bjnano.12.64

A review on nanostructured silver as a basic ingredient in medicine: physicochemical parameters and characterization

• Gabriel M. Misirli,
• Kishore Sridharan and
• Shirley M. P. Abrantes

Beilstein J. Nanotechnol. 2021, 12, 440–461, doi:10.3762/bjnano.12.36

• human serum albumin (HSA), fibrinogen and immunoglobulin (IgG), metallothionein (MT), and ceruloplasmin (CP), forming a protein corona (PC) during silver homeostasis [121][122]. The PC is a highly dynamic system and its composition dynamically changes over time, undergoing various transformations until

The impact of molecular tumor profiling on the design strategies for targeting myeloid leukemia and EGFR/CD44-positive solid tumors

• Nikola Geskovski,
• Nadica Matevska-Geshkovska,
• Simona Dimchevska Sazdovska,
• Marija Glavas Dodov,
• Kristina Mladenovska and
• Katerina Goracinova

Beilstein J. Nanotechnol. 2021, 12, 375–401, doi:10.3762/bjnano.12.31

Electrokinetic characterization of synthetic protein nanoparticles

• Daniel F. Quevedo,
• Cody J. Lentz,
• Adriana Coll de Peña,
• Yazmin Hernandez,
• Nahal Habibi,
• Rikako Miki,
• Joerg Lahann and
• Blanca H. Lapizco-Encinas

Beilstein J. Nanotechnol. 2020, 11, 1556–1567, doi:10.3762/bjnano.11.138

• vaccines [5][6]. Significant advances in self-assembled PNPs via protein engineering techniques have also been observed [7][8]. PNPs have successfully reached the clinic with nab-paclitaxel (Abraxane®), a PNP made of human serum albumin, which is being used for the treatment of metastatic breast cancer

Key for crossing the BBB with nanoparticles: the rational design

• Sonia M. Lombardo,
• Marc Schneider,
• Akif E. Türeli and
• Nazende Günday Türeli

Beilstein J. Nanotechnol. 2020, 11, 866–883, doi:10.3762/bjnano.11.72

• (e.g., human serum albumin) [28], gold nanoparticles [29] and superparamagnetic iron oxide nanoparticles [30]. This review aims to summarize (i) the different pathways to cross the BBB, (ii) the strategies that can be employed to increase nanoparticle BBB permeation without disrupting the BBB, as well

Luminescent gold nanoclusters for bioimaging applications

• Nonappa

Beilstein J. Nanotechnol. 2020, 11, 533–546, doi:10.3762/bjnano.11.42

• importantly, the Au-BSA NCs are stable under ambient conditions and retain their PL even after drying (Figure 1G,H) allowing for long-term storage [64]. Several other proteins including lysozyme, human serum albumin (HSA) and insulin have been used to prepare inherently luminescent AuNCs [65][66][67][68][69

• are presented. In each section representative early examples along with the recent examples are discussed. Biosensing and imaging of pathogens Chan et al. reported human serum albumin (HSA)-stabilized gold NCs (Au-HSA) for sensing Staphylococcus aureus (SA) and methicillin-resistant Staphylococcus

• : glutathione; NAC: N-acetylcysteine; MTU: 5-methyl-2-thiouracil). D) Structures of biomolecular ligands (HSA: human serum albumin, PDB ID IE78; BSA: bovine serum albumin, PDB ID 3VO3, and Lys: lysozyme, PDB ID 3WUN). E) Schematic representation showing Au-BSA NC synthesis. F) Absorbance and fluorescence

Rational design of block copolymer self-assemblies in photodynamic therapy

• Maxime Demazeau,
• Laure Gibot,
• Anne-Françoise Mingotaud,
• Patricia Vicendo,
• Clément Roux and
• Barbara Lonetti

Beilstein J. Nanotechnol. 2020, 11, 180–212, doi:10.3762/bjnano.11.15

• , the porphyrin photosensitizer was released and the size of the nanoobjects in solution increased (Figure 5d) [77]. For a combined photodynamic therapy/photothermal therapy (PDT/PTT) approach, indocyanine green (ICG) has been encapsulated in a protein, namely human serum albumin. First human serum

• ® use endogenous proteins such as human serum albumin (HSA) to take care of the drug traffic. In the case of PS and PDT, a first example has been described after a structural optimization of the PS (modified indocyanine) [131]. It is also noteworthy that human serum albumin has also been used in another

• albumin (HSA) is reduced and encapsulates ICG thanks to electrostatic interactions, then the disulfide links are reconstructed for carrier stabilization. This carrier is then glutathione-sensitive and its reduction under in vivo conditions enhanced the PDT efficiency [78]. The cleavage of ROS-responsive

Long-term stability and scale-up of noncovalently bound gold nanoparticle-siRNA suspensions

• Anna V. Epanchintseva,
• Julia E. Poletaeva,
• Dmitrii V. Pyshnyi,
• Elena I. Ryabchikova and
• Inna A. Pyshnaya

Beilstein J. Nanotechnol. 2019, 10, 2568–2578, doi:10.3762/bjnano.10.248

• of the clumping of AuNP-siRNA, we used the known property of albumin to increase the dispersion of AuNP suspensions [29]. We added 3% human serum albumin (HSA) (corresponding to 10% serum commonly used in the cell cultivation) to samples ×1 and ×10 (freshly prepared and stored for one or eight days

• . Experimental Materials and methods We used RNA phosphoramidite synthons, dithiothreitol (DTT), sodium citrate (Na3C6H5O7·2H2O, Sigma-Aldrich, USA); Cy-5.5 phosphoramidite (Primetech, Belarus); aurum hydrochloric acid (HAuCl4·3H2O, Aurat, Russia); human serum albumin (HSA, Reanal, Hungary); sodium chloride

• , storage for one day; 2, storage for eight days. Clusters of AuNP-siRNA in samples ×10 after two (A), three (В), and four (С) weeks. The same samples after the addition of 3% human serum albumin (HSA) in the DMEM medium (D, E, F). D–F – contrasting with uranyl acetate. Scale bars correspond to 100 nm. Data

Magnetic properties of biofunctionalized iron oxide nanoparticles as magnetic resonance imaging contrast agents

• Natalia E. Gervits,
• Andrey A. Gippius,
• Alexey V. Tkachev,
• Evgeniy I. Demikhov,
• Sergey S. Starchikov,
• Igor S. Lyubutin,
• Alexander L. Vasiliev,
• Vladimir P. Chekhonin,
• Maxim A. Abakumov,
• Alevtina S. Semkina and
• Alexander G. Mazhuga

Beilstein J. Nanotechnol. 2019, 10, 1964–1972, doi:10.3762/bjnano.10.193

• and human serum albumin coated iron oxide nanoparticles was observed by Mössbauer spectroscopy. Conclusion: This difference in magnetic behavior is explained by the influence of biofunctionalization on the magnetic and electronic properties of the iron oxide nanoparticles. The ZF-NMR spectra analysis

• functionalization of nanoparticles with bovine serum albumin (BSA) and human serum albumin (HSA) is one potential method to enhance their biocompatibility. There are several directions in the development of coating types: coating of the nanoparticles themselves [10], design of BSA microcapsules with iron oxide

Doxorubicin-loaded human serum albumin nanoparticles overcome transporter-mediated drug resistance in drug-adapted cancer cells

• Hannah Onafuye,
• Sebastian Pieper,
• Dennis Mulac,
• Jindrich Cinatl Jr.,
• Mark N. Wass,
• Klaus Langer and
• Martin Michaelis

Beilstein J. Nanotechnol. 2019, 10, 1707–1715, doi:10.3762/bjnano.10.166

• . Here, we tested doxorubicin-loaded human serum albumin (HSA) nanoparticles in the neuroblastoma cell line UKF-NB-3 and its ABCB1-expressing sublines adapted to vincristine (UKF-NB-3rVCR1) and doxorubicin (UKF-NB-3rDOX20). Doxorubicin-loaded nanoparticles displayed increased anticancer activity in UKF

• body distribution of its many substrates including drugs, xenobiotics, and other molecules. HSA nanoparticles may provide an alternative, more specific way to overcome transporter-mediated resistance. Keywords: ABCB1; cancer; doxorubicin; drug resistance; human serum albumin; nanoparticles

• resistance [6]. This includes various nanoparticle and liposome formulations of the ABCB1 substrate doxorubicin [7][8][9][10][11][12]. Here, we here investigated the effects of doxorubicin-loaded human serum albumin (HSA) nanoparticles in ABCB1-expressing neuroblastoma cells. HSA nanoparticles are easy to

Serum type and concentration both affect the protein-corona composition of PLGA nanoparticles

• Katrin Partikel,
• Robin Korte,
• Dennis Mulac,
• Hans-Ulrich Humpf and
• Klaus Langer

Beilstein J. Nanotechnol. 2019, 10, 1002–1015, doi:10.3762/bjnano.10.101

• significantly at low concentration levels but becomes constant when passing to higher serum concentrations. Corona formation is a very dynamic, competitive and time-dependent process. In the early stage, low-affinity proteins with high abundance in serum, for instance, human serum albumin (HSA), adsorb onto the

Review on nanoparticles and nanostructured materials: history, sources, toxicity and regulations

• Jaison Jeevanandam,
• Ahmed Barhoum,
• Yen S. Chan,
• Alain Dufresne and
• Michael K. Danquah

Beilstein J. Nanotechnol. 2018, 9, 1050–1074, doi:10.3762/bjnano.9.98

• powered by light on the market, representing the first major commercial use of dye-sensitized solar cells. In 2005, Abraxane™, which is a human serum albumin NP material containing paclitaxel, was manufactured, commercialized and released in the pharmaceutical market [41]. In 2014, there were about 1814

Comparison of four methods for the biofunctionalization of gold nanorods by the introduction of sulfhydryl groups to antibodies

• Xuefeng Wang,
• Zhong Mei,
• Yanyan Wang and
• Liang Tang

Beilstein J. Nanotechnol. 2017, 8, 372–380, doi:10.3762/bjnano.8.39

• modification with Traut’s reagent. These results are consistent with the findings that DTT modification introduces more sulfhydryl groups per mol human serum albumin (HSA) than treatment with Traut’s reagent [28]. The conjugating efficiency of antibody-to-nanosphere by Traut’s thiolation was low relative to

Au nanoparticle-based sensor for apomorphine detection in plasma

• Chiara Zanchi,
• Andrea Lucotti,
• Matteo Tommasini,
• Sebastiano Trusso,
• Ugo de Grazia,
• Emilio Ciusani and
• Paolo M. Ossi

Beilstein J. Nanotechnol. 2015, 6, 2224–2232, doi:10.3762/bjnano.6.228

• APO in blood plasma, as shown in Figure 7B. The kinetics of APO adsorption onto the gold nanostructured surface are expected to be strongly affected by matrix effects. In particular, the presence of proteins, such as human serum albumin, an abundant plasma protein that binds a wide range of drugs

Protein corona – from molecular adsorption to physiological complexity

• Lennart Treuel,
• Dominic Docter,
• Michael Maskos and
• Roland H. Stauber

Beilstein J. Nanotechnol. 2015, 6, 857–873, doi:10.3762/bjnano.6.88

• revealed a decrease of the collision efficiency (i.e., the overall number of collisions divided by the number of collisions that form an agglomerate) with an increasing protein (human serum albumin, HSA) concentration (Figure 1a). Intriguingly, they found that their colloid was stable at a point where

• the polymer shell), by live HeLa cells in the presence or absence of human transferrin (TF) and human serum albumin (HSA) in phosphate-buffered saline (PBS) medium. They studied the uptake of the NPs by quantitative confocal fluorescence microscopy. For comparison, they also studied the cellular

• in brackets. (d) Normalized Raman (d) and SERS (e) spectra of cystine (disulfide stretching mode region, alkaline solutions). Relative Gaussian areas for the six different S–S bond conformations are indicated in brackets. Adapted from [36]. Copyright 2014 Springer. Human serum albumin (HSA, PDB code

Coating with luminal gut-constituents alters adherence of nanoparticles to intestinal epithelial cells

• Heike Sinnecker,
• Katrin Ramaker and
• Andreas Frey

Beilstein J. Nanotechnol. 2014, 5, 2308–2315, doi:10.3762/bjnano.5.239

• ], and possible implications for the particle–cell interactions are considered. For example, it was shown that human serum albumin (HSA), BSA and fetal bovine serum can build a protein corona around NPs whereby the particles are stabilized against agglomeration and the colloid stability of the particle

Nanoencapsulation of ultra-small superparamagnetic particles of iron oxide into human serum albumin nanoparticles

• Matthias G. Wacker,
• Mahmut Altinok,
• Stephan Urfels and
• Johann Bauer

Beilstein J. Nanotechnol. 2014, 5, 2259–2266, doi:10.3762/bjnano.5.235

• Technische Universität Darmstadt, Technical Chemistry, D-64287 Darmstadt, Germany Merck KGaA, D-64287 Darmstadt, Germany 10.3762/bjnano.5.235 Abstract Human serum albumin nanoparticles have been utilized as drug delivery systems for a variety of medical applications. Since ultra-small superparamagnetic

• systemic exposition of patients with the carrier [6]. Human serum albumin (HSA) represents a promising candidate that binds a wide range of compounds with different physicochemical characteristics. In 2007, with Abraxane®, a first polymeric nanoparticle formulation consisting of this material has been

• time range. After freeze drying, only USPIO HSA hybrid particles prepared at an iron concentration of 15 µg/mg demonstrated significant agglomeration. Experimental Reagents and chemicals Human serum albumin (Fraction V) and glutaraldehyde 8% aqueous solution were purchased from Sigma (Steinheim

Effects of surface functionalization on the adsorption of human serum albumin onto nanoparticles – a fluorescence correlation spectroscopy study

• Pauline Maffre,
• Stefan Brandholt,
• Karin Nienhaus,
• Li Shang,
• Wolfgang J. Parak and
• G. Ulrich Nienhaus

Beilstein J. Nanotechnol. 2014, 5, 2036–2047, doi:10.3762/bjnano.5.212

• Marburg, Renthof 7, 35037 Marburg, Germany, Department of Physics, University of Illinois at Urbana-Champaign, 1110 West Green Street, Urbana, IL 61801, USA 10.3762/bjnano.5.212 Abstract By using fluorescence correlation spectroscopy (FCS), we have studied the adsorption of human serum albumin (HSA) onto

• four orders of magnitude. These variations can be understood in terms of specific Coulombic interactions between the proteins and the NP surfaces. Keywords: fluorescence correlation spectroscopy; human serum albumin; nanoparticles; protein corona; quantum dots; Introduction In recent years, both

• reliable in our hands. In our original FCS study [45], we investigated the adsorption of human serum albumin (HSA) onto carboxyl-functionalized, polymer-encased iron platinum nanoparticles (Fe–Pt NPs) with a hydrodynamic radius, RH, of 5–6 nm. We prepared HSA solutions of different concentrations in